Biotin-tagged proteins: Reagents for efficient ELISA-based serodiagnosis and phage display-based affinity selection
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چکیده
منابع مشابه
Biotin-tagged proteins: Reagents for efficient ELISA-based serodiagnosis and phage display-based affinity selection
The high-affinity interaction between biotin and streptavidin has opened avenues for using recombinant proteins with site-specific biotinylation to achieve efficient and directional immobilization. The site-specific biotinylation of proteins carrying a 15 amino acid long Biotin Acceptor Peptide tag (BAP; also known as AviTag) is effected on a specific lysine either by co-expressing the E. coli ...
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ژورنال
عنوان ژورنال: PLOS ONE
سال: 2018
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0191315